Purchase the type of mushroom you wish to grow. Place the mushroom gills-down on a piece of glass. Cover the mushroom and glass with a bowl to keep spores from escaping.
Leave the mushroom on the glass overnight. It helps to run a dehumidifier near the mushroom to dry it out more quickly. As the mushroom dries, spores will release and drop to the glass to be collected.
Place agar mix into a sterilized petri dish. All petri dishes are sterile upon purchasing. To re-sterilize an old petri dish, place it in a 1 to 9 bleach/water solution for two minutes, dip it in rubbing alcohol, then allow it to air dry.
Transfer the spores from the glass to the petri dish. Seal the dish and store it in a dark, warm place -- approximately 55 to 80 degrees Fahrenheit. The spores have successfully germinated when small strands branch out across the dish. This process can take anywhere from a few hours to a few days.