Wait approximately one and a half months to harvest lion's mane mushrooms that you grow at home. Those found in nature may take a few or up to 10 months to be ready for harvest.
Look for long spines on the mushrooms to know when they are ready for harvest. If the mushroom top is yellow or pink instead of white, it is past its prime. Another sign that it is too mature is a soft top.
Remove the lion's mane mushroom with extreme care. Use a sharp knife to cut it at the base. This type bruises quickly. Damaging the spines makes it susceptible to bacteria.