Use an herbicide containing glyphosate to kill a mulberry bush. Select an undiluted, water-soluble formula and review the manufacturer's instructions and safety warnings.
Spray the foliage of the mulberry bush from all sides. This is known as foliar treatment and is effective on bushes up to the height of 15 feet.
Cover plant evenly with herbicide but do not drench the leaves to the point of runoff. Do not use the chemical when there is a chance of rain within 6 hours of treatment. Avoid using glyphosate on very hot days or on plants that are severely drought-stressed. The best time to use the chemical is during August and September.
Repeat treatment after 7 to 10 days.
Cut down the dead bush with an axe and discard. Dig out the stump with hoe or shovel. If the stump is too large to dig out, use a stump grinder and grind it to a depth of 1 foot below the soil line.