Plant one muscadine grape vine every 10 feet along the fence line.
Choose a single, strong shoot to grow upward along the fence. Secure the shoot to the fence carefully with paper-covered wire or string. The University of Florida Extension recommends pruning off side shoots as they emerge but not removing the leaves growing directly on the trunk as the vine climbs the fence.
Prune the tip of the shoot when it reaches about 5 or 6 feet high to facilitate the growth of two side branches. These shoots will become the fruit-bearing vines, or cordons, and the upward-growing shoot will become the trunk.
Train the two new shoots to grow horizontally across the fence at about 5 or 6 feet above the ground by securing the vines to the fence gently with string or paper-covered wire ties. The vines can also be woven in and out of the fence mesh horizontally across the fence.