Remove muscadine grape seeds from the ripe fruit. Remove any attached pulp. Rinse with lukewarm water. Lay the seeds on a paper plate to dry, undisturbed for 24 hours.
Place damp peat moss into a plastic sandwich until it is half full. Drop the muscadine grape seeds into the baggie and seal it. Place the baggie in the refrigerator for three months.
Pour sterile potting soil into 4-inch pots until they are three-fourths full. Remove the muscadine grape seeds from the refrigerator after the three-month stratification period. Push two to three seeds ¼ of an inch into the soil of each pot.
Mist the soil with lukewarm water. Set the pots on a tray. Place the tray in an area that receives bright, indirect light. Maintain a temperature of approximately 75 degrees F during the germination process, which usually begins within two to three weeks.
Maintain bright light and moist soil conditions as the muscadine grape seedlings continue to grow. Transplant the seedlings into 6-inch pots when they outgrow the 4-inch pots. Transplant the seedlings outdoors after a full year of growth.
Dig holes large enough to accommodate the root balls of the muscadine grape seedlings. The leaves of the plants should remain above the soil line when positioned in the holes. Space the holes 20 feet apart.
Keep the soil moist at a 1-inch depth throughout the growing season. After the first year of growth, muscadine grapes will only require supplemental watering during drought.
Secure each of the muscadine grape plants with flexible cording attached to a stake. Set up a trellis behind each of the stakes to guide the muscadine grape plants upward as they grow.