Prune the side fruiting canes, called spurs, which produced muscadine grapes the previous season, to 4 or 5 inches. Leave vigorous vines with longer spurs, having four or more buds. These vines must be in soil that's high in nutrition and consistently moist to support a heavier grape production.
Thin the vine's fruiting spurs after their third harvest to prevent the plant from becoming crowded with canes. Don't remove all 3-year-old spurs, as that dramatically reduces crop yield. Select a few of the older canes to chop off every year.
Cut the tendrils if they encircle the grapevine's branches. Slash them with a sharp knife to keep them from killing the vine. Retain only the tendrils wrapped around the trellis.