Dig a 2-foot deep hole in the planting site. Fill the hole with water. If the water drains within four hours, the site has adequate drainage. If the water doesn't drain, choose a better draining site or amend the soil for drainage.
Spread 2 to 4 inches of composted manure or peat over the planting site to improve drainage. Work the compost into the top 8 inches of the soil.
Test the soil with a soil pH kit, which measures the acidity and nutrient qualities of the soil, following the directions included with the kit. Perform the test six months prior to planting. Muscadines prefer a pH range between 5.8 and 6.5.
Add agricultural lime to the planting site, using the amount recommended by the soil test. Cultivate the lime into the top 6 to 10 inches of the soil. Apply lime six months prior to planting.
Apply a fertilizer to the planting area two weeks before you plant. Follow the nutrient recommendations of the soil test to determine the type and amount of fertilizer necessary.