Watch your sprouted petunia seeds for their first true set of leaves. True leaves are the second set of leaves any seedling produces. The small oval leaves that emerge first are called seed or cotyledon leaves. Petunia leaves are elongated ovals that are slightly fuzzy.
Fill a plug flat with moist potting mix. Poke a small hole in the center of each cell with a pencil or chopstick.
Lift out a section of petunia seedlings with a fork. Work the utensil under the seedlings, scraping the bottom of the tray to avoid hitting the young roots.
Set the clump of seed-starting mix and seedlings on a piece of moist paper towel. Break up the clump gently with the point of the pencil or the tines of the fork. Discard the weakest seedlings and avoid handling the stems or roots of the petunias you are keeping.
Pick up one petunia by its leaves to avoid damaging it stem. It will not recover from stem damage at this stage. You can also catch the plant under its pair of leaves with the tines of the fork instead picking it up with your fingers.
Fold one end of the damp paper towel over the remaining seedling to keep them moist while you work.
Lower the petunia seedling straight into a hole in one of the cells. Set the plant so its leaves are just above the soil level. Ensure the hole is deep enough so the root doesn't double over in the bottom of the hole.
Firm the soil gently around the stem with your finger.
Repeat until you fill each plug cell with a petunia seedling.