Sprinkle diatomaceous earth on the mulch around the plants or on the soil. Diatomaceous earth, widely available where garden supplies are sold, is made of sedimentary rock crushed to a fine powder. The sharp particles wound the ants, causing dehydration and death. It isn't necessary to dust the ants directly with the powder. Next time the ants come around, the diatomaceous earth will stick to their bodies. Reapply the powder after irrigation and rain.
Select a commercially available organic insecticide for use in gardens. Products with pyrethrin and diatomaceous earth are for killing fire ant colonies, for example. Prepare the solution at the rates listed on the manufacturer's label. Pour the solution into the anthill.
Pour 1 to 2 gallons of boiling water into an anthill that fire ants have just built. You might have to flood the anthill again with boiling water if ant activity resumes. This method is 100 percent successful when the hot water manages to reach the queen. Do not use this option if the colony is next to vegetable plants. The hot water also kills roots.