Empty your Jacuzzi per manufacturer's directions.
Fill a bucket with 1 gallon of hot water. Pour 1/2 cup of bleach into the bucket.
Wear rubber gloves to prevent direct contact with skin-irritating bleach solution.
Wet the hot tub cleaning mitt with the diluted bleach solution. Scrub the interior of the hot tub with the bleach solution. Rinse the mitt as needed. Apply more bleach solution and wipe down the entire interior surface.
Dip an old toothbrush in the diluted bleach solution. Use the toothbrush to scrub around the jets and other grooved or textured area. Allow the bleach solution to remain on the Jacuzzi surface for 15 to 20 minutes to kill the mildew and sanitize the surface.
Dampen a clean cloth with water. Rinse the hot tub surface by wiping it off with the cloth.
Dry the Jacuzzi with clean cloths. Allow the interior to air dry overnight before refilling your hot tub.