Put on your safety goggles and dust mask. Mark the position and length of the groove on the wood with a pencil, using the straight edge to make sure it is perfectly straight. Use the T-square, if the groove needs to be perpendicular to the edge.
Place the edge of the biscuit joiner against the pencil line. Mark the joiner's edge with your pencil. Measure the distance between the mark and the pencil line. Place a corresponding pencil mark on the wood's opposite edge. Draw a line -- with a straight edge -- parallel to the one marking your groove.
Place your dimensional lumber on the wood so that it is flush with the parallel line. Fasten each end to the wood with the adjustable clamps. This is your jig.
Place the biscuit joiner against the jig to ensure the edge of the blade lines up with first pencil line. Adjust the jig if necessary.
Set the biscuit joiner's blade to the groove's depth. Keeping the biscuit joiner against the jig, make your cut in a single, fluid motion.