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How to Detect Anions in Soil

Soil containing high levels of salts adversely affects plant growth. Excess salts can be toxic, disturb nutrient uptake, lower water availability by affecting the osmotic process and change the balance of ions in the plant. Ions are particles with either a positive of negative charge. Anions are negatively charged ions. The anions that are the most common causes of high salinity are nitrates, sulfates, chlorides, bicarbonates and hydroxides. Conducting an electrical conductivity (EC) test identifies the presence of high levels of salts in the soil.

Things You'll Need

  • Electrical conductivity meter
  • Deionized water
  • Lab cleaning tissue that does not leave lint
  • Standard solution with an electrical conductivity close to that of the soil
  • Soil sample
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Instructions

    • 1

      Clean the electrodes (probes) of the electrical conductivity meter by rinsing them with deionized water. Tap water contains ions from soil and pipes. During an electrical conductivity test, the water ions can change places with other ions in the soil and change the results. Blot the probes dry with a lab cleaning tissue.

    • 2

      Choose a standard solution with a known electrical conductivity close to the EC that you believe your soil will be. A standard solution is a mixture of evenly distributed substances with a known concentration. Change the setting on the EC meter to "calibrate." There are multiple ways to calibrate meters. Follow the manufacturer's instructions for calibrating the EC meter with the standard solution.

    • 3

      Change the EC meter from "calibrate" to "measure." Insert the probe into the solution, and wait until the meter stabilizes to verify the electrical conductivity of the standard solution.

    • 4

      Clean the probes again, rinsing them with deionized water. Blot the probes dry with a lab cleaning tissue.

    • 5

      Insert the probes into the soil sample in the measure mode. The anions increase the conductivity of the water in the soil. Allow time for the meter reading to stabilize. If the meter does not automatically take the temperature of the sample into account, adjust the knob on the meter according to manufacturer's directions. Temperature is important for accurate readings. As the temperature increases, so does the resistance of the conducting metal in the probe.