Water the petunias deeply whenever the soil 1/2 inch below the surface feels dry to the touch if the petunias are growing in a container or whenever soil about 1 inch below the surface feels dry if the petunias are growing in the ground. Mites are most problematic on drought-stressed plants.
Pinch or cut off portions of the petunia that are severely infested with mites if the infestation is largely concentrated in these areas. Bag and dispose of the infested portions you remove. This pruning is particularly beneficial where mites have spun webs.
Spray the petunias down with a forceful spray of water every few days to knock these pests off and alleviate the dusty conditions mites prefer. Make sure the spray reaches leaf undersides, as mites tend to cluster on lower leaf surfaces.
Mix 5 tablespoons of neem oil with 1 gallon of water. You can also use another horticultural oil. While most oils use this dilution rate, check the label for specifics. You can also use another type of horticultural oil, if you prefer.
Put the mixture in a spray bottle or garden sprayer and spray the petunia with the mixture. Make sure you cover the plant's leaves thoroughly, though not to the point of runoff, and reach leaf undersides. The oil will only kill mites it directly contacts, so you may need to do this each week until the mites are under control.