Cut a groove or notch on the sassafras tree, using a hatchet. The cut should penetrate 1/2 inch on small sassafras trees and up to 2 inches on mature sassafras trees. Small sassafras trees need narrow grooves 1 to 2 square inches, whereas larger sassafras trees must have grooves 6 to 8 square inches. Cutting grooves into the sassafras disrupts its ability to transport nutrients and water.
Continue making grooves or notches around the tree. Cuts should be placed around the entire tree trunk.
Place a piece of plastic underneath the sassafras to protect grass or landscape plants.
Apply a herbicide to the cuts that contains 2,4-D + 2,4-DP, using a hand-held sprayer. Soak the cuts with the herbicide, but wipe off excess herbicide running down the tree to protect grass or landscape plants.
Reapply the herbicide in five days if the tree isn't exhibiting signs of dying, such as stunted growth, leaf discoloration or discolored bark.