Purchase tree seedlings that are inoculated with a truffle species. These seedlings can be purchased from a few online retailers. Pick a tree species that is adapted to growing in your climate. In the Southern United States, pecan truffles thrive growing on the roots of pecan trees, while in the Northwest, black truffles are commonly grown on hazelnut and oak trees.
Select your site for truffle growing. Most truffle species require mild climates that do not experience extreme heat or cold. The soil of your site must drain well and, because truffles are sensitive to the acidity of the soil, must have a pH of around 8. Lime can be used to correct the pH level of your soil.
Plant your tree seedlings in late winter but before spring vegetation begins to appear. Trees typically take three to five years to mature to a point where truffle growth will be possible. The truffles will kill the grass around the base of the tree when they are ready for harvest. This is the where the big time investment comes into truffle growing, but your patience will be rewarded.
Harvest your truffles. Truffles are tubers, which means they grow underground. Some truffieres use specially trained dogs to sniff out truffles growing underground, but because you planted your tree specifically for truffles, you can simply dig for them at the base of your trees.