Place the manometer in the room with one end of the manometer tube extended to the outside through the room's wall.
Measure the level of the fluid in the tube both inside and outside the room. If the levels are equal, record an equal air pressure measurement for inside and outside.
Place an exhaust fan in the room and shut the door. Turn on the exhaust fan to create and test for negative static pressure. The manometer should show a higher reading on the section of the tube inside the room. The difference between the fluid level of the manometer inside and outside the room is the amount of negative pressure within the room.